ACRO基于成熟的TR-FRET技术平台自主开发了一系列通用型工具产品，包含：

经Eu或FA标记的链霉亲和素（Streptavidin-Eu/ Streptavidin-FA）：可结合任意生物素化分子（蛋白、肽、小分子、核酸等）

经Eu或FA标记的抗标签抗体（anti-His、anti-Fc、anti-Flag等）：适配标签融合蛋白，项目兼容性更广

经Eu或FA标记的二抗类（anti-Human IgG / anti-Mouse IgG）：识别Fc区域，可用于抗体及Fc标签蛋白的检测需求

TR-FRET体系搭建专用反应缓冲液：经系统验证及优化，完美适配TR-FRET体系需求

Toolbox可为药物开发和研究提供更高的灵活性与可塑性，基于不同的研究方案及策略，您可以自行选择靶点并搭配合适的Toolbox，快速完成体系搭建，支持多种应用场景下的实验需求。

👍🏼依托于成熟的重组蛋白开发平台，ACRO可提供数千种蛋白产品，覆盖众多热门适应症靶点，蛋白纯度及活性均经验证，让您轻松完成TR-FRET体系搭建！

使用Toolbox搭建的TR-FRET体系示意图

更多Toolbox产品持续开发中，敬请期待~

ACRO研发的Toolbox产品可以支撑搭建更高信噪比的TR-FRET体系，适用于各种不同灵敏度需求的项目，可保证良好的实验重复性。

• 结合体系验证

Details of the assay components are provided in the table above. Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6), at 20 ng/well, can bind to the Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear detection range of 8-0.03125 μg/mL.

Details of the assay components are provided in the table above. Biotinylated Human PD-1 Protein, Avitag,His Tag (Cat. No. PD1-H82E4), at 16 ng/well, can bind to the Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a linear detection range of 10-0.0195 μg/mL.

Details of the assay components are provided in the table above. Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6), at 50 ng/well, can bind to the Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear detection range of 5-0.01 μg/mL.

• 抑制体系验证

Details of the assay components are provided in the table above. An inhibition Assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc, Avi tag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4 resulting in a typical IC₅₀ of 1.774 μg/mL.

Details of the assay components are provided in the table above. An inhibition Assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc, Avi tag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4 resulting in a typical IC50 of 9.13 μg/mL.

在相同实验体系下，ACRO的Toolbox产品表现出更高的信噪比、更优的灵敏度和良好的重复性。

• 结合体系验证

The example below compares two Donor-Acceptor pairs across ACRO, Supplier 1 and Supplier 2 in a protein-protein interaction assay between Biotinylated Human PD-1 Protein, Avitag,His Tag(ACRO,Cat. No.PD1-H82E4) and Monoclonal Anti-Human PD-1 Antibody, Human IgG4. Regardless of the Donor-Acceptor pair used, ACRO’s S/B significantly outperforms that of Supplier 1 and Supplier 2.

• 抑制体系验证

Details of the assay components are provided in the table above. An inhibition assay was performed to evaluate the interaction between Biotinylated Human PD-1, Fc, Avi tag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) using Monoclonal Anti-Human PD-1 Antibody, Human IgG4. Using the ACRO’s Toolbox to build the system provides excellent signal-to-noise ratio and repeatability, enabling the complete inhibition curve of the sample to be plotted.

您可根据验证需求选择合适的实验组分并匹配相应的Toolbox，Donor和Acceptor可以灵活搭配。

1. 所选的Donor和Acceptor前体不能相同。例如，若选择Streptavidin Protein Europium chelate (for TR-FRET)（Cat. No. FRT-TD09）作为Donor，则不能选择Streptavidin Protein FA-Labeled (for TR-FRET)（Cat. No. FRT-TA01）做为Acceptor，两者会与反应体系中的同一组分结合，导致体系搭建失败。

2. 所选择的Donor和Acceptor不能相互结合。例如，若选择Monoclonal Anti-His Tag Antibody, Mouse IgG1 FA-Labeled (for TR-FRET)（Cat. No. FRT-TA05），则不能选择Monoclonal Anti-Mouse IgG Antibody Europium chelate (for TR-FRET)（Cat. No. FRT-TD03），否则在没有其他实验组分的情况下两者也会相互结合，产生非特异信号。

3. 所选择的Donor和Acceptor只能分别与其他实验组分之一相结合，不可与其他任何组分发生反应，否则将会造成非特异反应，导致实验失败。

想更快、更轻松地获得可直接使用的TR-FRET 试剂盒？我们可为您提供多款现货产品，覆盖多种适应症热门靶点，并支持专业定制，让您的实验真正做到“开箱即用、一步到位”！

>>点击图片了解TR-FRET试剂盒产品

探寻机制，验证筛选？ACRO高品质靶点蛋白与抗体同样值得信赖！

TR-FRET试剂盒优异性能的背后，是ACRO对核心原材料质量的严格把控。我们同样提供结构天然、构象完好、生物活性高、质控严格的BCMA、VEGF165等靶点蛋白与对应抗体原材料，适用于多种应用场景，助您夯实研究基础。

TR-FRET直播课——TR-FRET技术赋能药物筛选与机制研究