Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.

As well as horseradish peroxidase kits, the Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.

Horseradish Peroxidase (HRP) Label

Horseradish peroxidase is a 44kDa glycoprotein with 6 lysine residues which can be conjugated to labeled biomolecules using Lightning-Link®. The enzyme label can be visualized by chromogenic reactions; for example diaminobenzidine (DAB) in the presence of hydrogen peroxide (H₂0₂) is converted in to a water insoluble brown pigment. Other substrates which can be used to measure horseradish peroxidase activity include ABTS, TMB and TMBUS.

Click here for other labels available in the Lightning-Link® range.

Manufacturing - Lightning-Link® Horseradish Peroxidase

The technology is fully scalable (μg to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.

Please contact us if you require any additional information and to discuss your bulk or custom conjugation requirements (info@innovabiosciences.com).

To find out more:

• Contact technical enquiries – technical.enquiries@innovabiosciences.com
• Read our simple guide to antibody labeling.
• Look at the Lightning-Link® video to visualize how easy it is to make high quality antibody conjugates.
• Review the frequently asked questions (FAQs) section to further familiarize yourself with the Lightning-Link® technology.

Western blotting data comparing conjugated and unconjugated antibodies

	conjugated	unconjugated
Primary antibody	Goat Anti-NQO1	Goat Anti-NQO1
Target	quinone reductase 1	quinone reductase 1
Sample lysate	human kidney	human kidney
Primary antibody working concentration	0.00425 µg/ml	0.1 µg/ml
Secondary antibody used	no (direct conjugation)	yes
Exposure time (min)	10	10
Primary antibody source	Everest Biotech, Cat no: EB05370
Western blot analysis

	conjugated	unconjugated
Primary antibody	Goat Anti-GFAP	Goat Anti-GFAP
Target	GFAP	GFAP
Sample lysate	mouse brain	mouse brain
Primary antibody working concentration	0.185 µg/ml	0.5 µg/ml
Secondary antibody used	no (direct conjugation)	yes
Exposure time (min)	3	3
Primary antibody source	Everest Biotech, Cat no: EB07478
Western blot analysis

by InnovaBiosciences